Evaluating the Effect of Microbial Stimulation and Oxidative Stress on Increasing β-Carotene Production in Blakeslea trispora

Document Type : Research Paper


1 Faculty of Life Sciences, Islamic Azad University, North Tehran Branch,Tehran

2 Department of Biotechnology, Iranian Research Organization for Science and Technology, Tehran, P. O. Box 3353-5111, Iran.

3 Department of Microbiology, North Tehran Branch of Islamic Azad University, 1667934791 Tehran, Iran



β-carotene is a lipophilic pigment that belongs to the carotenoid family and is the most widely utilized carotenoid in the industry which is produced by plants and microorganisms as a secondary metabolite. Blakeslea trispora is one of the most significant industrial sources of β-carotene production among microorganisms. The present study aims to investigate the effect of K. rhizophila as microbial stimulant and butylated hydroxytoluene (BHT) as oxidative stress on increasing β-carotene production in B.trispora. B. trispora was cultivated in the production medium with and without butylated hydroxytoluene (BHT), and after 24 hours 10 % K. rhizophila cultures with 1011 CFU/mL was added in each medium and incubated for another 4 days. The percentage of carotenoid isomers produced in each sample was determined using high-performance liquid chromatography (HPLC). K. rhizophila and BHT, each alone, could increase the carotenoid production by 2.3 and 2.4 times (respectively) compared to the control. The maximum concentration of carotenoids (793 mg/L) was found in samples containing both BHT and K.rhizophila, representing a 7.5-time increase over the control sample. HPLC analysis of carotenoids showed two main peaks including β-carotene and γ-carotene. The main carotenoid was β-carotene found in all samples, followed by a lesser amount of γ-carotene. Overall, microbial stimulant and oxidative stress were effective strategies for increasing β-carotene production in this microorganism.


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