Document Type : Research Paper
Authors
1
School of Public Health, Tehran University of Medical Sciences Tehran, Iran
2
Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran
3
Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
4
Immunology, Asthma, and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran
5
School of Public Health Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Cockroaches are one of the most important causes of asthma and allergies in residential areas. Sensitization to cockroach allergens is an established risk factor for asthma among populations. In this study, Cockroach hole bodies and feces of all two species were separately dried, homogenized, and centrifuged after one day of staying in PBS (phosphate-buffered saline). The protein concentration of the samples was determined using the Bradford method. The supernatant was prepared for injection into 20 mice, and after four weeks of subcutaneous injection, the ELISA test was used to determine the antibody titer against the injected antigen. After detection of OD (overdose), an SDS-PAGE test was performed to determine the molecular band and molecular weight of the peptides, and then a Western blot test with anti-mouse IgE conjugated HRP (Horseradish Peroxidase) for IgE in mouse serum was prepared. Comparison of the cockroach OD with the rest showed that more antibodies, both for feces and for the body, were produced in the mouse serum than in other cockroaches. A Western blot test also showed that the body of this cockroach produced IgE antibodies in the serum of mice. The molecular weights of body and feces samples of German cockroaches by SDS-PAGE were 85, 75, and 100 kDa, respectively, and 75, 35, 60, and 60 kDa, respectively, for the body and feces of the brown-banded cockroach. The weight of IgE protein bands (antibody) produced in mouse serum by western blot varied from 60 to 220 kDa. The upper titer of the whole body and the Cockroach feces of Supella longipalpa were observed in comparison with the other cockroaches, and the IgE antibody band of the Supella hole body was observed, which indicates the risk of allergy to this cockroach.
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