Document Type : Research Paper
Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
The increasing prevalence of diabetes is one of the most important health challenges worldwide. Serine/threonine-protein kinase-4 (STK4) is important in various cellular processes, with particularly in diabetes. Cannabis is a plant species that contains various medicinal compounds. This study aimed to examine whether the six compounds found in Cannabis extract can inhibit the STK4 protein that is present in diabetes. The crystallized structure (.pdb format) of Cannabis extract compounds were obtained from the PubChem database and used as ligands. Using the mm2 method, the ligand's structure was optimized. AutodockVina was employed to assess the ligand's effectiveness as an inhibitor against the active site of STK4 chain A and B. The results generated were analyzed and evaluated using Discovery Studio v16.1.0 software. Toxicity prediction of the best inhibitor was done by ProTox-II. The best affinity was obtained against 6YAT -chain A by -9.1 kcal/mol. The highest diversity of links was also reported in Ligand C with 6YAT -chain A. Hydrogen bonds established with 6YAT -chain A against Tyrosine: 104, Arginine: 245 and Phenylalanine: 244, indicating the effectiveness of delta(9)-Tetrahydrocannabinolic acid against chain A of 6YAT. Toxicity prediction showed that all pharmacokinetic parameters of ligand C molecule are in the acceptable range. Our study can provide valuable information about newly identified inhibitors for the treatment of diabetes. The findings of this study indicated that delta (9)-Tetrahydrocannabinolic acid molecule could be used as a novel STK4 inhibitor in the future studies.