Document Type : Research Paper
Department of Horticulture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
Department of Pharmacognosy and Traditional Pharmacy, Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran
Plant developmental cell, Ghadir Research Center, Ahvaz, Iran
Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Baqiyatallah University of Medical Sciences, Tehran, Iran
Essential oils of different plants are widely used in the pharmaceutical and food fields. In order to assess the composition of the essential oils, study antimicrobial properties, and compare the effect of essential oils of two Cupressus species with imipenem as a carbapenem antibiotic on wound infections, the chemical composition of essential oils of Cupressus sempervirence L. and C. funebris Endl. were obtained by Clevenger apparatus and analyzed by chromatography–mass spectrometry (GC/MS). The essential oil efficiency was estimated at a rate of 0.3%. In total 10 compounds were identified from the essential oils of each species. The results showed that C. sempervirens mainly consisted of 21.5% totarol, 15.54% delta-3-carene, 14.37% α-pinene and 11.78% phenanthrene and C. funebris mainly contained 24% α-cedrol, 18.11% naphthalene, 12.96% α-pinene, 10.05% delta-3-carene and 9.3% α-cedrene. The results of the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), showed that the essential oils of two species could inhibit the growth of most type strains of Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (DSM 50071T). Time-killing assay revealed that compared to imipenem, essential oils further reduced the bacterial colony growth after 24 hours’ incubation. On the other hand, essential oils of two species of Cupressus, have shown more efficient bactericidal effects versus imipenem.