Once the acute lymphoblastic leukemia cells that need L-asparagine are exposed to L-ASNase, they die because of the limitations of L-asparagine. The globally rising rate of ALL also requires extraordinary efforts to discover new microorganisms with high L-ASNase production and efficiency. The aim of this study is the high amount of L-ASNase production. After isolation, the L-ASNase production was optimized using the response surface methodology and the central composite design. Then, in-silico studies were predicted for the L-ASNase-producing gene. In this study, Bacillus velezensis was isolated as an L-ASNase producer from slaughterhouse effluent using the M9 medium. The optimization process further illustrated Tween 20, glucose, temperature, and L-asparagine, which were more significant for L-ASNase production. Based on statistical prediction by response surface methodology, more enzyme activity (7.11 U/mL) could be realized at 0.6% Tween 20, 1.7% glucose, 55°C temperature, and 1.8% L-asparagine. The in-silico studies also established that the binding site is located at the N-terminal domain and the active site flexible loop. Additionally, it contained Thr36, Ala47, Tyr50, Glu84, Asp117, Thr116, Met142, Lys189, Thr193, and Thr192 as the conserved and functional residues in L-ASNase. It was concluded that B. velezensis SE114 produced L-ASNase type II in the present study. The statistical optimization results also showed that Tween 20, glucose, temperature, and L-asparagine were significant variables affecting the L-ASNase production. In addition, temperature and L-asparagine had noteworthy interactions.
Hozoorbakhsh, F., Ghiasian, M., Ghandehari, F., Emami-Karvani, Z., & Khademi Dehkordi, M. (2023). Optimization of L-asparaginase type II produced by Bacillus velezensis SE114. Microbiology, Metabolites and Biotechnology, 6(2), 1-16. doi: 10.22104/mmb.2024.6548.1129
MLA
Fereshteh Hozoorbakhsh; Mozhgan Ghiasian; Fereshte Ghandehari; Zarrindokht Emami-Karvani; Maryam Khademi Dehkordi. "Optimization of L-asparaginase type II produced by Bacillus velezensis SE114". Microbiology, Metabolites and Biotechnology, 6, 2, 2023, 1-16. doi: 10.22104/mmb.2024.6548.1129
HARVARD
Hozoorbakhsh, F., Ghiasian, M., Ghandehari, F., Emami-Karvani, Z., Khademi Dehkordi, M. (2023). 'Optimization of L-asparaginase type II produced by Bacillus velezensis SE114', Microbiology, Metabolites and Biotechnology, 6(2), pp. 1-16. doi: 10.22104/mmb.2024.6548.1129
VANCOUVER
Hozoorbakhsh, F., Ghiasian, M., Ghandehari, F., Emami-Karvani, Z., Khademi Dehkordi, M. Optimization of L-asparaginase type II produced by Bacillus velezensis SE114. Microbiology, Metabolites and Biotechnology, 2023; 6(2): 1-16. doi: 10.22104/mmb.2024.6548.1129
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