Assessment of the probiotic isolates features found in breast milk

Articles in Press

Document Type : Research Paper

Authors

1 Department of Biotechnology Faculty of New Sciences and Technologies Semnan University Semnan, I.R.Iran

2 Dep. for Biology, Faculty of Basic Science, Semnan University, IRAN

3 Assoc. Prof. Shakiba Darvish AliPour Ph.D. in Microbiology Faculty of Biotechnology University of Semnan Semnan, I.R.Iran

Abstract

The probiotics in breast milk can help children restore optimal growth. This study aimed to separate and identify probiotic microorganisms from human milk and evaluation of vitamin B production by these microorganisms. Biochemical tests were used to identify nine aerobic and 81 microaerophilic isolates from 34 breast milk samples; Probiotic traits were characterized for auto-aggregation, co-aggregation, hydrophobicity, acidity tolerance, lysozyme, bile salts, and papain treatment. Furthermore, in some of the probiotic isolates, the production of the Vitamin B group was detected by thin layer chromatography.

Breastmilk samples were collected from 34 healthy women with an average age of 27.5 ± 6.5 years. Eighty-one colonies were isolated under microaerophilic conditions based on different morphological characteristics. Fungi, gram-positive and gram-negative bacteria were composed 36%, 34%, and 30% of the isolates respectively. Two isolates with strong probiotic potential were molecularly examined and named Enterococcus faecalis (SUBC 156), and E. faecalis (SUBC 169). The highest resistance to acidity (18%) and bile salt (-1) was displayed by SUBC169, while SUBC156 survived lysozyme treatment and had a hydrophobicity of 56%. SUBC153 and SUBC156 produced vitamin B6 in a milk-containing MRS medium. Breast milk is recommended because of its probiotic qualities. Identifying those strains may confirm the true beneficial effect of probiotics in infant nutrition.

Keywords

Main Subjects

Microbiology, Metabolites and Biotechnology

Articles in Press, Accepted Manuscript
Available Online from 12 August 2024

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