Antibacterial Potential of Lucilia Sericata excretions/secretions in bovine cutaneous wounds

Articles in Press

Document Type : Research Paper

Authors

1 Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran

2 Department of Animal and Poultry Science and Fisheries, Agricultural Institute, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran

3 Iranian Research Organization for Science and Technology: Tehran,

4 Agricultural Sciences and Natural Resources University of Khuzestan, Iran

Abstract

In the last decade, many patients worldwide have treated their wounds with maggots, so many doctors recognize maggot therapy as an influential supplement in traditional medicine. In this study, the excretions/secretions (ES) of Lucilia sericata larvae that have antibacterial effects on bovine chronic wounds bacteria and fibroblast cells were examined. Microbial cultures were taken from samples obtained from cows with chronic wounds, and the effect of larval ES was evaluated on them. The viability of bovine fibroblast cells was assessed using MTT assay. The results showed that the minimum inhibitory concentration (MIC) of larval ES on Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli was 40 mg mL-1. The results of the disk diffusion MIC experiment reveal that larval ES was able to control the growth of bacteria from bovine chronic wounds. The MTT assay showed that 0.1, 0.05, and 0.025 mg mL-1 of larval ES did not affect the survival of bovine fibroblasts, while concentrations similar to or greater than 0.25 mg mL-1 caused a significant decrease in the proliferation and viability of the fibroblast cells (p<0.05). In conclusion, the maggot L. sericata ES was able to control bovine chronic wound bacteria in vitro, but the use of more than 0.25 mg mL-1 can produces side effects on living cells. In vivo studies are recommended for the evaluation of the maggot L. sericata ES on cutaneous bovine wounds.

Keywords

Main Subjects

Microbiology, Metabolites and Biotechnology

Articles in Press, Accepted Manuscript
Available Online from 30 November 2024

Files

Share

How to cite

Statistics